壳寡糖锌配合物的制备及抑菌活性

摘要

探索壳寡糖锌配合物的制备工艺及评价其抑菌活性.根据Box-Behnken试验原理,以壳寡糖(chitosan oligosaccharide,COS)与Zn2+配比、体系pH值、反应温度及时间为影响因素,以对金黄色葡萄球菌和大肠杆菌的抑制活性作为响应值,进行壳寡糖锌(COS-Zn2+)制备工艺响应面优化分析.结果发现,COS-Zn2+制备条件对其抑菌活性影响从大到小依次为:COS与Zn2+配比>反应温度>反应时间>体系pH值,进而获得最佳制备工艺参数:pH5,反应时间2 h,糖盐比例1:1(质量比),反应温度45℃.在此参数条件下,制备COS-Zn2+对金黄色葡萄球菌和大肠杆菌的抑菌圈直径为33.08 mm和32.23 mm,与建立模型的预测值基本相符.研究结果可为海洋壳寡糖资源生物活性开发及应用,提供一定的参考与理论基础.%The present experiment was conducted to investigate the preparation of chitosan oligosaccharide-zinc (COS-Zn2+)complex and focused primarily on its antibacterial activity.According to Box-Behnken experimen-tal principle,the response surface methodology was performed,with the ratio of COS to Zn2+,pH,reaction tem-perature,and time as the experimental factors,versus the inhibitory activities against S.aureus and E.coli as response value.The results indicated the influence of preparation factors on antibacterial activity was as follows:the ratio of COS to Zn2+,temperature,time,and pH,respectively.Then,the optimum preparation conditions were obtained,which the value of pH was 5,the reaction time was 2 h,the ratio of COS to Zn2+was 1:1(mass ratio),and the temperature was 45℃,respectively.Under these conditions,the inhibition diameters of pre-pared COS-Zn2+on S.aureus and E.coli were 33.08 mm and 32.23 mm,respectively,which were almost ac-corded with the predicted data.These results could provide a reference and theoretical basis for the development and application of marine chitosan oligosaccharide resources.