冬凌草甲素对MG-63转移的抑制效应和分子机制研究

摘要

Objective To determine metastasis-inhibiting effect of oridonin on MG-63 cells and to explore its mechanisms.Methods After MG-63 cells were incubated with different concentrations of oridonin,the metastasis ability was analyzed with experiment of chemotaxic migration,the mRNA expressions of VEGF and MMP-9 in MG-63 cells were analyzed by RT-PCR and the changes of concentrations of VEGF and MMP-9 in supernatant were analyzed by ELISA.Results Oridonin effectively inhibited the metastasis of MG-63 cells in a concentration-time dependent way.Oridonin effectively inhibited the expressions of VEGF and MMP-9 in MG-63 cells and supernatant in a concentration-time dependent way.Conclusion Oridonin can inhibit the metastasis of MG-63 cells effectively in a concentration-dependent manner and the molecular mechanism may relate to oridonin down-regulating VEGF and MMP-9 expressions.%目的 确定冬凌草甲素是否具有抑制MG-63细胞转移的效应及其分子机制.方法 用不同浓度的冬凌草甲素作用于MG-63细胞,用体外驱化运动试验检测MG-63细胞运动驱化能力的变化;用RT-PCR检测MG-63细胞中血管内皮生长因子(VEGF)和基质金属蛋白酶(MMP-9) mRNA表达的变化;用ELISA方法检测MG-63细胞上清VEGF和MMP-9浓度的变化.结果 (1)体外驱化运动试验证明冬凌草甲素能以浓度依赖性方式抑制MG-63细胞转移; (2)冬凌草甲素以浓度依赖性方式抑制MG-63细胞及其上清中VEGF和MMP-9的表达.结论 冬凌草甲素具有抑制MG-63细胞转移的能力,其机制可能与药物下调VEGF和MMP-9有关.