比较分析了分子标记核DNA ITS1序列和Cyt b基因序列在凤鲚遗传多样性分析和凤鲚与其他鲚属鱼类（以刀鲚为例）间亲缘关系研究中的特征。研究发现凤鲚其 ITS1序列的 GC 含量（71．19％）显著高于Cyt b基因序列（42．96％），ITS1序列的单倍型多样性（0．900）和核苷酸多样性（0．00997±0．01116）也显著高于Cyt b基因序列（0．400/0．000369±0．000469）。基于ITS1序列所得的凤鲚与刀鲚间的MCL和K2P遗传距离可明确区两种鲚属鱼类，与Cyt b基因序列的结果相一致。通过ML和UPGMA系统树的构建发现，凤鲚和刀鲚分别形成独立的分支。本研究显示ITS1序列与Cyt b基因序列所得结果一致性较好，可考虑将ITS1序列作为凤鲚的遗传多样性分析及其与其他鲚属鱼类间亲缘关系研究中有效的分子标记。%The DNA ITS1 and Cyt b gene were sequenced in tapertail anchovy Coilia mystus as a very economically important fish in Yangtze River estuary to reveal ITS 1 sequence characteristics and potential utilization for genetic diversity and kinship analysis for this species and other members in genus Coilia . The results showed that there was higher GC content in ITS1 sequence (71 .19% ) than that in Cyt b gene sequence (42 .96% ) .Also ,the haplotype and nucleotide diversity of ITS1 sequence (0 .900/0 .00997 ± 0 .01116) was higher than that of Cyt b gene sequence (0 .400/0 .000369 ± 0 .000469) .Both MCL and K2P genetic distances of ITS1 were found at interspecies level between C .mystus and C .nasus ,which were coincident with those of Cyt b gene sequences . The ML and UPGMA trees constructed with ITS1 sequences and Cyt b gene sequences revealed that C . mystus and C . nasus formed the separate monophyletic groups ,respectively .Hence ,the consistency of analyses between ITS1 sequence and Cyt b gene sequence were good ,indicating that the ITS1 sequence was used as a good genetic marker for genetic diversity and kinship determination for C .mystus and other Coilia species (e .g .,C .nasus) .
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