大黄鱼精子稀释液初步研究

摘要

研究了淡水鱼生理盐水、海水鱼生理盐水、Holtfreter溶液、Ringer溶液4种配方的精子稀释液在常温和4℃保存大黄鱼精子的效果。试验结果表明，4种稀释液在4℃保存效果均优于常温。4℃，添加葡萄糖后的4种稀释液保存效果均优于未添加稀释液。其中添加葡萄糖的 Holtfreter溶液效果最好，在4℃最大保存时间可超22 h ，明显优于其他稀释液。用Holtfreter稀释液保存的精子人工授精的受精率可达73．3％。%The effects of 4 diluents on sperm quality in large yellow croaker (Pseudosciaena crocea) were studied under room temperature and low temperature (4 ℃) .The results showed that the diluents showed better preservation under low temperature (4 ℃ ) than that under room temperature , especially the diluents containing glucose better than the one without glucose .Holtfreter solution had the best effect in all the four diluents ,and the maximum preservation time of over 22 hours under the low temperature , much longer than under the room temperature .The fertilization rate of 73 .3% was observed in the eggs fertilized by the sperm preserved in Holtfreter solution .