模拟真空辐照环境对Ames和SOS标准菌株的影响

摘要

利用空间低能综合辐照实验设备对Ames标准菌株TA97,TA98,TA100,TA102和SOS显色反应标准菌株PQ37进行真空辐照处理,研究航天器内真空辐照环境对微生物的影响.通过鼠伤寒沙门氏菌回复突变试验(Ames试验)和SOS显色法对航天器内的真空辐照环境的致突变作用进行生物学评价.研究结果显示,模拟航天器的真空辐照环境具有诱变性,且对微生物的生理代谢有一定的影响.%Ames Salmonella TA97, TA98, TA100, TA102 and E.coli PQ37 were treated in vacuum and irradiation environment using a space combined low energy irradiation test equipment developed by Beijing Institute of Spacecraft Environmental Engineering. The influence of vacuum and irradiation environment in spaceships on microbe was studied. Back mutation test (Ames test) and SOS chromotest test were carried out on salmonella to evaluate the mutation effect of vacuum and irradiation environment in spaceships. The results of both SOS chromotest and Ames test indicated that spaceship environment can induce mutations and has certain effect on physiological metabolism of microbe.