The goat estrus|associated oviductual glycoprotein(gEGP), which is an important secretary protein in oviduct, is directly associated with the zona pellucida of the ovulated oocyte and with the preimplantation embryo development. To elucidate the physiological function and fundamental mechanism,a goat genomic library, in which plague titer is 1^15×10+6 pfu/ml, was constructed. Then, with a probe derived from bovine EGP cDNA, the genomic library was screened and a 18 kb fragment containing the gEGP gene was obtained. After PCR with the primers based on the ovine EGP cDNA, two fragments of gEGP gene were obtained and then sequenced. The predicted exon sequence of gEGP gene shows 99 6%, 96 3%, 89 2%, 88 4%, 88 0%, 87 4%, 84 3%, 74 8% homology with the EGP cDNA of sheep, cow, pig, baboon, rhesus, human, hamster, mouse, respectively. Like the other members of EGP gene family, gEGP gene also has many properties of the chitinase super family, and demonstrates the phylogenetic and functional relativity of EGP family with chitinase family.
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