氧化型低密度脂蛋白诱导血管平滑肌细胞凋亡的机理研究

摘要

To investigate the mechanism involved in the apoptosis of vascular smooth muscle cells induced by oxidized low density lipoprotein(OX LDL),cultured human vascular smooth muscle cells derived from umbilical artery were exposed to OX LDL.The apoptotic cells were determined by deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL).The gene expressions of p53,p21,c fos and c jun were quantified by reverse transcription polymerase chain reaction(RT PCR).The contents of p53,p21,c fos and c jun were analyzed by immunochemical staining.The apoptotic vascular smooth muscle cells were found following treatment with OX LDL.The levels of p53,p21,c fos mRNA and protein were increased in vascular smooth muscle cells exposed to OX LDL.However,c jun expression remained constant.These results indicated that OX LDL could upregulate the expression of p53,p21 and c fos,inducing the apoptosis in vascular smooth muscle cells.