不同配比头孢哌酮钠/舒巴坦对革兰阴性杆菌体外抗菌效果影响

摘要

Objective To explore the differences of antibacterial effect in vitro between two different concentrations of Cefoperazone-Sulbactam (2: 1 and 1: 1) 75/30 μg (SCF105)and 75/75 μg (SCF150) upon gram-negative bacilli. Meth-ods Susceptibility test of 86 strains of clinical isolated gram-negative bacilli was conducted with K-B method using SCF105 and SCF150 respectively and the diameter of inhibition zone were measured. The Susceptibility results of two kinds of suscepti-bility sheets were analyzed and the antimicrobial results on the four common gram-negative bacillus ( Pseudomonas aeruginosa, e. Coli, klebsiella pneumoniae and acinetobacter baumannii) was explored. Results For all the 86strains, susceptibility rate and intermediate rate SCF 150 disc were 53. 5% and 26. 7%, which were significantly higher than that with SCF105 disc;the resistance rate with SCF disc was 19. 7%, significantly lower than the resistance rate of SCF105 disc (P<0. 01). Wilcox on signed rank sum test for the four kinds of clinical common gram negative bacterium ( Pseudomonas aeruginosa, Escherichia co-li, Klebsiella pneumoniae and Acinetobacter baumannii) was used and sensitivity rate with SCF150 disc was higher than the result of SCF105 disc, and the difference was significant, E. Coli (P<0. 05);For all strains and four gram negative bacteri-a, the inhibitor diameter of SCF105 disc was smaller than that of SCF150 disc (P<0. 05), and especially in E. coli (P<0. 05). Compared with the inhibitor diameter 4 kinds gram-negative bacilli, there were significant differences between SCF105 of E. Coli and Pseudomonas aeruginosa and acinetobacter baumannii);meanwhile, there were significant differences between SCF150 of E. coli and 3 other kinds (P<0. 05). Conclusion AS far as antibacterial effectiveness of gram-negative bacilli in vitro is concerned, composition ratio of 75/75μg Cefoperazone-Sulbactam is superior to that of 75/30μg Cefoperazone-Sul-bactam.%目的 探讨头孢哌酮钠/舒巴坦75/30 μg(SCF105)和75/75 μg(SCF150)两种配比药敏纸片对革兰阴性杆菌的体外抗菌效果. 方法 用纸片扩散法检测SCF105和SCF150两种药敏纸片对临床分离的86株革兰阴性杆菌的抑菌环直径,比较药敏结果并分析两种药敏纸片对4种常见革兰阴性杆菌(铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌及鲍曼不动杆菌)的抗菌效果. 结果 本研究检测86株革兰阴性杆菌的敏感率和中介率SCF150纸片均高于SCF105纸片(P<0. 01);SCF150纸片全部菌株的耐药率低于SCF105纸片(P<0. 01). 对4种革兰阴性杆菌的敏感率SCF150纸片均高于SCF105纸片,其中差异最明显的是大肠埃希菌(P<0. 05). 对全部菌株及4种革兰阴性杆菌抑菌环直径SCF105纸片均小于SCF150纸片(P<0. 05). 4种革兰阴性杆菌抑菌环直径两两比较显示,SCF105纸片中大肠埃希菌与2种菌株(铜绿假单胞菌及鲍曼不动杆菌)以及SCF150纸片中大肠埃希菌与其他3种菌株抑菌环直径比较差异均有统计学意义(P<0. 05). 结论 成分配比为75/75 μg的头孢哌酮钠/舒巴坦药敏低纯对革兰阴性杆菌的体外抗菌效果更优.