目的：观察GATA-3和T-bet在妊娠期高血压疾病患者胎盘及血清中的表达情况,探讨GATA-3和T-bet与妊娠期高血压疾病的相关性。方法选择子痫前期及子痫孕妇47例,根据病情将其分为轻度子痫前期组11例,重度子痫前期组18例,子痫期组18例；另选择同期正常妊娠晚期孕妇20例作为正常妊娠组。采用免疫组织化学法与酶联免疫吸附试验法测定各组胎盘及血清中GATA-3与T-bet的表达水平并进行比较。结果在妊娠期高血压疾病患者及正常妊娠者胎盘及血清中GATA-3和T-bet均有表达。与妊娠期高血压疾病各组比较,正常妊娠组胎盘及血清中GATA-3水平最高,T-bet水平最低,差异均有统计学意义( P<0．01)；妊娠期高血压疾病各组胎盘及血清中GATA-3随着病情加重表达量逐渐降低,T-bet随着病情加重表达量逐渐增高,各组间两两比较差异均有统计学意义(P<0．01)。结论胎盘及血清中GATA-3和T-bet的表达与妊娠期高血压疾病的发病及严重程度密切相关。%Objective To observe the expression of GATA-3 and T-bet in both placenta and blood serum of patients with hypertensive disorder during pregnancy and to investigate the relationship between the GATA-3, T-bet and the hyperten-sive disorder during pregnancy. Methods A total of 47 patients who suffered from preeclampsia or eclampsia disorder during pregnancy were divided into three groups: gestational hypertension group (11 patients), severe preeclampsia group (18 pa-tients) and eclampsia group (18 patients). 20 normal pregnant women were chosen as normal pregnancy group. Immunohisto-chemistry and ELISA were used to examine the placenta and blood serum expression of GATA-3 and T-bet. Results GATA-3 and T-bet were expressed in placenta and serum of patients with hypertensive disorders during pregnancy and normal preg-nancy. The levels of GATA-3 in the placenta and serum were the highest and T-bet was the lowest in normal pregnancy group, and differences between the normal pregnancy group and hypertensive disorders group were significant (P<0. 01). Placenta and blood serum of GATA-3 in the 3 groups of gestational hypertension patients decreased as the condition deteriorated, but T-bet concentration increased as the condition deteriorated, and the differences among the three groups showed significant differ-ence (P<0. 01). Conclusion The expressions of GATA-3, T-bet in placenta and blood serum are closely related to hyper-tensive disorders during pregnancy and degree of severity.
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