Objective To observe oligodendrocytes gliomas chromosome 1p/19q deletion and Ki-67 protein expression,explore new molecular markers forecasting chemosensitivity of oligodendrocytes glioma.Methods 31 oligodendrocytes glioma specimens as the experimental group,13 cases adjacent normal brain tissue of oligodendrocytes glioma as a control group,the Ki-67 protein detected by immunohistochemistry and 1p/19q detection detected by fluorescence in situ hybridization.Results Compared with control group,the experimental group had 14 cases of Ki-67 expression with positive rate 45％;1p/19q deletions was not detected in the control group,1p/19q loss for 13 cases(41.9％) in the experimental group of 31 cases of oligodendroglioma glioma specimens,1p alone missing for 2 cases(6.4％),19q alone missing for 1 case(3.2％).Ki-67 protein expression and the 1 p/19q loss was significant correlation(P＜0.05).Conclusion Ki-67 protein expression in the gliomas may be as thepotential molecular markers of chemosensitivity oligodendrocytes gliomas.%目的 观察少突胶质细胞瘤1号染色体短臂(1p)和19号染色体长臂(19q)联合杂合性缺失(LOH)与Ki-67蛋白表达的相关性,探索预测少突胶质瘤化疗敏感性的分子标记物.方法 少突胶质瘤肿瘤组织标本31例作为实验组,少突胶质瘤瘤旁正常脑组织标本13例作为对照组,免疫组化方法检测两组Ki-67蛋白的表达,荧光原位杂交技术检测1p/19q的缺失情况.结果 对照组有1例Ki-67表达呈现阳性(7.7％),实验组有14例Ki-67表达呈现阳性(45.0％),两组阳性率差异有统计学意义(P＜0.05)；对照组无1p/19q缺失,实验组有13例(41.9％)1p/19q联合缺失,其中1p单独缺失2例(6.4％),19q单独缺失1例(3.2％).Ki-67蛋白表达与1p/19q联合缺失显著相关(r=0.403,P＜0.05).结论 Ki-67蛋白表达是预测少突胶质瘤化疗敏感性的潜在分子标记物.
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