MMEJ-KO:a web tool for designing paired CRISPR guide RNAs for microhomology-mediated end joining fragment deletion

摘要

Dear Editor,Clustered regularly interspaced short palindromic repeats associated protein(CRISPR/Cas)systems are convenient and versatile tools for genome editing.Directed by a guide RNA(gRNA),the Cas nuclease produces double-strand break(DSB)at the target site,generally resulting in small base insertion/deletion mutations by the non-homologous end joining(NHEJ)DNA repair.