快速免疫色谱测试法与免疫荧光法检测登革病毒IgG抗体的比较

摘要

Aim To compare IgG antibody tests for diagnosis of dengus fever with Denger Fever IgM and IgG Rapid immunochromatographic Test(DRIT, Panbio, Australia) and Indirect Immunofluorescent Assay(IIF). Method DRIT was followed the kit's direction, and the IgG positive result(only IgG was compared in the sutdy) was determined only appearing two amaranth lines,one is in control region, another is in IgG region. Antigen slide in IIF was prepared with dengus virus type 2 isolated in the laboratory and it was performed routinely. Results 56 sera were collected from the suspected patients with dengus fever. Among these specimens, IIF positive rate was 53.6 % (30/56), and DRIT was 46.4 % (26/56), there were no significant difference between the two tests. In comparison with IIF however, when testng 68 sera collected from the asymptom population, positive rate was 54% (37/68)for IIF, and 33.8 % (23/68) for DRIT, there existed significant difference between the two methods( X2= 9.39, P ＜ 001). Conclusion There is no significant difference bwtween IIF and DRIT in test the IgG antibody of the patents. DRIT is a simpl, rapid, and easy method to the determination, but IIF is a ideal routine technique for use in both of the patients and the asymptom population since it's rather economic, and more sensitive.%目的 应用登革热快速免疫色谱测试法(Dengue Fever Rapid Immunochromatographic Test，DRIT)与间接免疫荧光法(Indirect Immunofluorescent Assay，IIF)检测登革病毒IgG抗体进行比较。方法DRIT按说明书操作，在Control线区出现紫红色线的前提下在IgG线区出现紫红色线表明IgG抗体阳性；IIF抗原片系应用本室分离的登革Ⅱ型病毒感染C6/36细胞制备，操作按常规进行。结果检查临床疑似登革热病人血清56份，IIF检测阳性30份，阳性率53.6％。DRIT检查阳性26份，阳性率46.4％，两法符合率91.07%，X2=1.50，P＞0.05。检测无症状人血清68份，IIF检查阳性37份，阳性率为54.4％。DRIT检查阳性23份，阳性率为33.8％，两法符合率73.53％，X2=9.39，P＜0.01。结论检测病人血清两法无显著性差别，具有操作简单，时间较短，结果易观察等优点。IIF成本较低，检测病人或无症状人群IgG抗体均较敏感，仍不失为理想的常规方法。