动态检测特异性免疫复合物在脑囊虫病治疗过程的意义

摘要

目的 了解特异性免疫复合物在脑囊虫病治疗过程中的意义。方法 用双单抗夹心ELISA法检测特异性免疫复合物：采用抗人IgG、IgM单抗包板，来捕获由血清提取的免疫复合物中的特异性IgG、IgM型免疫复合物，通过酶标抗囊虫单抗结合物显色，测其OD值。结果 IgG型免疫复合物在1疗程后含量较治疗前明显升高（P＜0.05）以后各疗程则显著下降(P＜0.01)；IgM型免疫复合物的含量治疗前与1疗程后相比差别不显著，但随着疗程增加其含量逐渐下降(P＜0.01)；各疗程的临床反应加重发生率以第1疗程最高(P＜0.005)。结论 IgG型免疫复合物可以作为治疗反应、疗效考核的依据。%Aim To observe the dynamic changes of specific immune complexover all courses of drug administration of patients with neurocysticercosis. Methods By using a double mono-cloned antibody sandwich ELISA assay the specific immune complex was detected.Employed anti-human IgG, IgM McAb as coated material, and HRP labeled anti-cysticerci McAb as recognizing system, specific immune complexes that extracted in sera were monitored. Results The immune complex level of IgG class rose significantly after the 1st course (P＜0.05), and then gradually dropped off (P＜0.01). On the other hand, that of IgM class did not change significantly after 1st course of treatment. It gradually declined with advancing course of drug administration (P＜0.01). The incidence rate of clinical worsening after 1st course of treatment was the highest one among all courses (P＜0.005).Conclusion This study indicates that immune complex level of IgG class is associated with the clinical worsening and the efficacy of therapy.