In order to determine the efficacy of inactivated rabies vaccine (Hep-Flury-dG) strain, we carried out an expending experiment in a village of Guangzhou. The information of all the dogs vaccinated in this research was collected. On day 0 before immunization and day 21 post-immunization, the blood samples of 66 dogs were collected. The serum were separated and the antibody against rabies virus were detected by ELISA. Results showed that seroconversion were 26% before using the inactivated vaccine, while was 83% after immunization. Statistical analysis indicated that there was statistical significance between antibody levels before and after immunization. The gender of the dog was not a factor that could affect the antibody level , while the age was the key factor. Thus, the inactivated rabies vaccine could offer protection against rabies virus from dog's immune system.%目的 检测狂犬病基因工程灭活疫苗(Hep-Flury-dG株)注射犬只后的免疫效力.方法 在广州市增城区某村进行狂犬病基因工程灭活疫苗(Hep-Flury-dG株)环境释放试验,登记该村所有家养犬的信息并存档,同时进行疫苗免疫.随机采集免疫前血清样品66份,免疫后21 d仍获得血清样品66份,使用ELISA方法检测样品的抗体水平.结果 免疫前该村犬只抗体阳转率为26％,免疫后21 d抗体阳转率达到83％.经统计分析表明免疫前后抗体水平差异具有统计学意义；性别不是影响抗体水平变化的因素,但年龄可影响抗体水平变化,成犬抗体水平的增加明显高于幼犬.结论 狂犬病基因工程灭活疫苗(Hep-Flury-dG株)免疫犬只后可提供足够的保护力,有效保护犬只免受狂犬病病毒感染,因而预防狂犬病从犬-犬或犬-人的传播；为农村地区家养犬建立免疫档案,监控犬只抗体水平,使犬群间形成狂犬病抗体阳性率达到70％以上的坚强免疫带,是预防农村地区狂犬病发生的一种可行、有效的方法.
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