The purpose of this research was to investigate a new method of transhepatic injection to establish primary E-chinococcus multilocularis (E. m) model in liver of mouse. The 120 healthy female mice of 12-week-old were randomly divided into three groups as follows: transhepatic injection through skin incision and musculi abdominis, laparotomic transhepatic injection, and percutaneous transhepatic injection (40 in each group). By giving intraperitoneal injection with 20% Urethane, every mouse was injected with E. m tissue suspension respectively through three different operational methods to establish mimic primary E. m model in liver of mouse. Results showed that the survival rates of mice of the three groups were 85%, 75% and 80%, respectively (P>0. 05); the E. m infection rates in liver of mouse were 70. 6%, 70. 0% and 62. 5%, respectively (P>0. 05) ; and the incidence of inadvertent puncture of pleural were 0% , 0% and 25% , respectively (P<0. 01). A primary E. m model in liver of mouse can be successfully established by the method of transhepatic injection through skin incision and musculi abdominis, which was not only simplified the operation of laparotomic transhepatic injection but also reduced the incidence of inadvertent puncture of pleural following percutaneous transhepatic injection by transhepatic injection through skin incision and musculi abdominis.%目的 探讨新型肝穿刺方法建立原发性肝多房棘球蚴(Echinococcus multilocularis,E.m)小鼠模型.方法 将120只12w龄健康雌性小鼠随机分为切开皮肤经腹壁肌层肝穿刺接种、开腹肝穿刺接种和经皮肝穿刺接种3组,每组40只.20%乌拉坦腹腔注射麻醉后,分别对3组小鼠进行肝脏注射E.m组织混悬液,制备模拟原发性肝多房棘球蚴小鼠模型.结果 3组小鼠的存活率依次为85%、75%、80%(P>0.05),肝脏E.m感染率依次为70.6%、70.0%、62.5%(P>0.05),胸腔误穿率依次为0%、0%、25%(P<0.01).结论 切开皮肤经腹壁肌层肝穿刺接种可成功制备模拟原发性肝多房棘球蚴小鼠模型,既简化了开腹接种的操作,又减少了经皮肝穿刺误穿胸腔的发生率.
展开▼
