青岛市售养殖海水虾中副溶血性弧菌的分离及耐药性分析

摘要

目的 对青岛市售养殖海水虾中副溶血性弧菌进行分离鉴定,并对分离菌株进行耐药性分析.方法 以常规培养法分离,全自动细菌鉴定仪进行鉴定,PCR扩增法检测毒力基因,K-B法进行药敏试验.结果 样品的副溶血性弧菌检出率为78.1% (50/64),所有菌株均不含tdh基因,其中3株菌含trh基因,所有菌株对头孢拉啶耐受,96%菌株对氨苄西林和阿莫西林耐受,部分菌株对头孢呋新钠、链霉素、四环素、土霉素和复方新诺明耐受,少量菌株出现耐受3类抗生素以上的多重耐药性.结论 青岛市售养殖海水虾中副溶血性弧菌存在较严重的污染和一定程度的耐药情况.%The Vibrio parahaemolylicus strains in farmed marine shrimps from Qingdao markets were isolated and identified and the antimicrobial resistance of these isolates was tested. Presumed V. parahaemolylicus in shrimps were isolated by traditional culture method and identified by automatic bacterial identification system. The virulence genes were determined by PCR amplification and the antimicrobial resistance was performed by K-B method. A total of 50 (78. 1%) V. parahaemolylicus strains were isolated from 64 shrimp samples. The tdh gene was absent in all the isolates and trh gene was found in three of them. All isolates were resistant to cephradine and 96% of them were resistant to ampicillin and amoxycillin. Some isolates were resistant to cefuroxime sodium, streptomycin, tetracycline, oxytetracycline, and sulphamethoxazole/trimethoprim. A few isolates exhibited multidrug-resistance against at least three kinds of antibiotics. The results indicated that the farmed marine shrimps from Qingdao markets are highly contaminated by V. parahaemolylicus and the isolates have antimicrobial resistance to a certain extent.