新疆伊犁地区尼勒克县人群泡球蚴感染病例调查及nad2、cob基因片段分析

摘要

We investigated the endemic situation of alveolar echinococcosis (AE) in Nileke County,Yili Prefecture,Xinjiang and its genetic diversity of Echinococcus multilocularis.Human AE cases in the county were retrospectively investigated including their location and surrounding ecological conditions.Sequence detection and analysis of nad2,cob gene fragments of AE mtDNA from patients was used to identify genotype variation.Results showed that a total of 48 AE cases were diagnosed and the first AE patient was identified in 1989 in the county.The 45.8％ of AE cases were found in the recent 5 years (2011-2015) and annual prevalence was 1.7 per 100 000.The patients were distributed along the Kashgar River,particularly intensive in Wulasitai Township.The 38.6％ of the patients were aged arranged 35-44 years old,64.6％ were male and 95.8％ were farmers and herdsmen.AE cases were confirmed further and there was only one haplotype by sequencing analysis from 11 AE clinical patients in the county.It suggests that Nileke County is AE foci,and alveolar echinococcosis with sequences conserved is an emerging disease in the county.%目的 了解新疆伊犁地区尼勒克县泡球蚴感染确诊病例人群分布及病原遗传特征.方法 收集和调查尼勒克县境内人群泡球蚴感染病例相关数据,并利用分子生物学技术对收集到的患者病灶肝组织样本,针对线粒体had2和cob基因片段开展病原分子检测和分析.结果 截止2015年底共收集到泡球蚴感染患者资料共计48例,首例感染病人确诊于1989年.近5年确诊病例占到所有病例数的45.8％,年均确诊率为1.7/10万.感染人群沿喀什河流域两岸分布,在乌拉斯台乡尤为集中.患病人群中35-44岁占到38.6％,男性占64.6％,农牧民占95.8％.通过对11份病例样本检测had2、cob基因片段进一步证实为泡球蚴感染,序列自身比对完全同源.结论 泡球蚴在尼勒克县属于新发传染病.包括尼勒克县在内的伊犁河谷地带为泡球蚴的自然疫源地,该县境内的泡球蚴病原核酸序列较为保守单一.