首次自褐家鼠粪便标本中检出香港海鸥菌

摘要

目的 了解褐家鼠粪便标本中是否携带香港海鸥菌,并分析其耐药特征及分子分型.方法 2015年6月-2016年5月,利用笼捕法于广州市南方医院及其周边居民区捕获褐家鼠,其粪便样本经增菌后接种改良头孢哌酮MacConkey琼脂(CMA)平板培养分离可疑菌株,经生化试验及16S rRNA测序确认.采用K-B法进行药物敏感性测定,并运用多位点序列分型进行分子分型分析.结果 共捕获褐家鼠191只,并在2份褐家鼠粪便标本中检出香港海鸥菌,检出率为1.05％.两株菌16S rRNA的测序结果与香港海鸥菌标准株HKU1的一致性达100％.药敏试验显示两株分离株均对头孢菌素类抗生素及利福平耐药.多位点序列分型结果显示两株菌分别为2个新的ST型:ST-163和ST-164.结论 褐家鼠粪便中存在香港海鸥菌污染.褐家鼠与人类生活关系密切,可能为人类感染的的另一潜在来源.%In order to investigate whether Laribacter hongkongensis could be detected in stool samples of Rattus norvegicus in the wild,Rattus norvegicus were trapped alive in an urban community of Guangzhou,China over a period of one year from June 2015 to May 2016,and their stool samples were examined for the presence of L.hongkongensis strains.Isolates were identified based on phenotypic characteristics and 16S rRNA sequence analysis,and were examined for their susceptibility to 19 antimicrobial agents.Further typing of the isolates was performed using multi-loci sequence typing (MLST) analysis.A total of 191 R.norvegicus were trapped alive.L.hongkongensis was identified and successfully isolated from two samples,representing a prevalence of 1.05 ％.Although the two isolates possessed similar phenotypic characteristics and have no base difference of 16S rRNA gene,they constituted two new distinct sequence types (STs),ST-163 and ST-164.This is the first report that L.hongkongensis can be detected in the intestinal tract of R.norvegicus.Results suggest that R.norvegicus could serve as carriers of L.hongkongensis and therefore could be another potential source of infection.