AIM: To evaluate the effect of vascular endothelial growth factor ( VEGF ) on the ovarian tissues during transplantation. METHODS: Twenty regularly cycling rats were randomly divided into 2 groups: the rats in group A received fresh autologous ovarian transplantation without VEGF administration ( n = 10 ); the rats in group B received fresh autologous ovarian transplantation with VEGF administration ( n = 10 ). Four weeks after transplantation, the ovarian tissues and sera of the rats were collected for histological examination and determination of the hormone levels. RESULTS: The serum concentration of estradiol in group B was significantly higher than that in group A ( P < 0.05 ). Four weeks after transplantation, we found that the ovarian tissues were significantly developed, and the average size of the ovaries in group B was significantly larger than that in group A ( P <0.05 ). CONCLUSION: The ovarian tissues with VEGF administration develop better than those without VEGF administration. VEGF can increase the graft vascularity and the percentage of surviving tissues.%目的:初步探讨血管内皮生长因子(VEGF)在卵巢组织自体移植中的应用价值.方法:将健康成熟未交配过的20只雌性SD大鼠随机分为2组,A组去势后自体异位移植新鲜卵巢组织,B组去势后自体异位移植经VEGF处理过的新鲜卵巢组织.移植后饲养4周,观察动情周期的恢复情况和卵巢组织的形态学变化,测定血清中的激素水平.结果:B组大鼠的雌二醇水平明显高于A组,其差异有统计学意义(P<0.05);B组移植卵巢组织的体积明显大于A组,其差异有统计学意义(P<0.05);对形态正常的各级卵泡统计分析,生长卵泡数目在各组之间的差异没有统计学意义.结论:在卵巢组织移植过程中使用VEGF,可以减少卵巢组织的缺血再灌注损伤,有助于促进卵巢组织功能的恢复.
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