黑龙江省大豆品种对大豆疫霉根腐病抗性评价及抗性基因推导

摘要

Phytophthora root and stem rot, caused by Phytophthora sojae, is an economically important disease of soybean ( Glycine max) in Heilongjiang Province of China. Forty - four soybean cultivars were evaluated for their responses to P. sojae race 1,3,4,5,9, 13,44 and 54. Results showed that resistant and intermediate cultivars to the eight races accounted for 29.5% ,40.9% ,43.2% ,36.8% ,45.5% ,31. 8% ,and 72.7% respectively. Forty -four soybean cultivars elicited 37 different reaction types with the eight P. sojae races. Hefeng - 44 and Suinong -12 were deduced to have gene Rpslc or the combination of Rpsla and Rpslc,Rpsc and Rps6, Rpslc and Rpsl. Heihe -32 was deduced to have the combination of gene Rpsla and Rpsld. Other cultivars could carry this new single gene or combination of these two genes respectively. It was suggested that there were abundant' germplasmresources that resisted Phytophthora root and stem rot of soybean in Heilongjiang Province.%采用下胚轴伤口接种法,利用课题组在黑龙江省分离鉴定的8个大豆疫霉根腐病生理小种(race1、race3、race4、race5、race9、race13、race44、race54)对黑龙江省曾经大面积推广种植的44个大豆品种进行接种鉴定.结果表明,对8个生理小种分别有45.5％、29.5％、40.9％、43.2％、36.8％、45.5％、31.8％、72.7％表现抗性或中间类型,共产生37种反应型；通过基因推导,合丰-44,绥农- 12可能含有Rps1c基因或Rps1a+Rps1c、Rps1c+ Rps6、Rps1c+ Rps7的基因组合；黑河-32可能含有Rps1a+Rps1d的基因组合,其它品种可能含有新的抗病基因或基因组合,表明黑龙江省存在丰富的抗大豆疫霉根腐病的种质资源.