人脑胶质瘤干细胞TGF-32mRNA的表达及意义

摘要

目的 检测人脑胶质瘤干细胞(BGSCs)中转化生长因子-β2(TGF-β2) mRNA的表达水平,推断其在胶质瘤免疫治疗中的作用. 方法 选取中国医科大学第一医院神经外科自2008年10月至2009年1月间手术切除的脑胶质瘤标本8例,培养出BGSCs,免疫荧光染色检测肿瘤细胞球CD133、GFAP和TU-20的表达；RT-PCR检测BGSCs中TGF-β2 mRNA的表达,并与对应的原代分化脑胶质瘤细胞进行比较. 结果 肿瘤细胞球CD133呈阳性表达；分化于肿瘤球的细胞可表达GFAP和TU-20; RT-PCR检测发现TGF-β2 mRNA在BGSCs中的表达水平远高于原代分化胶质瘤细胞,差异有统计学意义(t=3.619,P=0.009). 结论 BGSCs中TGF-β2 mRNA呈高表达,这可能是胶质瘤TGF-β2高分泌、产生免疫逃逸并耐受各种常规治疗的决定性因素.%Objective To determine the mRNA expression of transforming growth factor β2 (TGF-β2) in human brain glioma stem cells (BGSCs),and investigate its effect onimmunotherapy of glioma. Methods BGSCs were grown from 8 specimens resected from glioma from October 2008 to January 2009,then,they were isolated and identified.The expressions ofCD133,GFAP and TU-20 in the tumor cell spheres were detected by immunofluorescence staining; the TGF-β2 mRNA expression in BGSCs was detected by RT-PCR,and then,this result was compared with that of respective differentiated glioma cells. Results Positive expression of CD133 was noted in the tumor cell spheres,and GFAP and TU-20 could be expressed in the tumor cell spheres. A quantitative determination exhibited higher TGF-β2 mRNA expression in BGSCs than that in their counterparts of primary cultured glioma cells,which was statistically significant (t=3.619,P=0.009). Conclusion High TGF-β2 mRNA expression is noted in BGSCs,which might be the main producer of overproduction of TGF-β2 in gliomas leading to immune escape and resistance to current therapies.