2种中药单体对念珠菌的体外药敏研究

摘要

目的 探讨人参茎叶皂甙、三七总皂甙、氟康唑、伊曲康唑体外抗念珠菌的敏感性.方法 采用CLSI公布的M-27A方案微量液体稀释法分别测定氟康唑、伊曲康唑、人参茎叶皂甙、三七总皂甙对60株临床分离念珠菌的体外敏感性.结果 氟康唑、伊曲康唑、人参茎叶皂甙、三七总皂成对受试菌的MIC50分别为0.25,0.25,7.812和15.625mg/L,MIC90分别为64,16,250和250mg/L.60株念珠菌临床分离株中有10株对伊曲康唑、人参茎叶皂甙、三七总皂甙交叉耐药.结论 人参茎叶皂甙、三七总皂甙在体外对念珠菌均有不同程度抑制作用,CLSI M27-A方案可用于对中药抗念珠菌活性的评价.%Objective To compare the susceptibilities of Candida albicans cultured with ginseng stems and leaves saponins,Panax Pseudoginseng,fluconazole and itraconazole. Methods Susceptibilities of 60 candida isolates cultured with fluconazole,itraconazole, ginseng stems and leaves saponins and Panax Pseudoginseng was determined by M27-A Microdilution Method based on CLSI.Results The MIC50 and MIC90 of Candida albicans to fluconazole ,itraconazole, ginseng stems and leaves saponins and Panax Pseudoginseng were 0.25 rng/L,0.25 mg/L,7. 812 mg/L, 15. 625 mg/L (MIC50), and 64 mg/L, 16 mg/L,250 mg/L,250 mg/L (MIC90) ,respectively. Ten of the total 60 isolates showed cross-resistances to itraconazole, ginseng stems and leaves saponins and Panax Pseudoginseng. Conclusions Ginseng stems and leaves saponins and Panax Pseudoginseng have similar antifungal effects on Candida albicans. CLSI-M27-A could be used to evaluate the anti-candida activity to the components of medicinal plant as a standard method.