超滤管在陈旧生物检材中的应用

摘要

目的：探讨millipore超滤管滤过方法对陈旧生物检材DNA分型检验的应用价值。方法将23份陈旧血样分别剪取同样合适大小的血片3组，标为A、B、C组，磁珠法提取DNA，分别用80μL、80μL、20μL洗脱液洗脱得到模板DNA，其中A、C组模板直接扩增，B组用millipore超滤管滤过浓缩后扩增。PCR产物用AB3130xL基因分析仪检测，GeneMapperID V3.2软件进行自动分型。所得实验数据用SPSS软件分析处理。结果 A组没有1例样本扩出全部STR基因座，B组有18例样本扩出全部STR基因座，C组有11个样本扩出全部STR基因座。结论应用millipore超滤管滤过方法可以明显提高陈旧生物检材的DNA分型成功率。%Objectve To investigate the application value of ultraifltration with millipore ifltration method test of old biological samples DNA. Methods 23 old blood samples separately were cut blood piece equally suitable size of three groups, labeled A, B and C group. DNA was extracted by magnetic bead methed and get DNA template with 80μL, 80μL, 20μL elution solution, respectively, then the DNA template in group A and C take right amount directly ampliifed, group B with milipore ultraifltration ifltration condensed amplication. PCR products were detected by ABI3130xl genetic analyzer and analyzed by GeneMapperID V3.2 software. The date were analyzed by using SPSS software. Results No samples were ampliifed for all STR loci in group A. There were 18 cases in group B samples amplified all STR loci and 11 samples in group C. Conclusion Application of millipore ultrafiltration method can signiifcantly improve the old biological samples DNA classiifcation success rate.