目的：观察牙龈成纤维细胞的肝细胞生长因子(HGF/SF)的分泌方式。方法：培养牙龈成纤维细胞；制作该细胞胶原网架(FPCLs)，将FPCLs分组，从FPCL形成后的0～7d，每天从3个不同角度测量FPCL的直径缩小值。结果：不含血清培养液(SFM)组与SFM+rHGF组FPCL的收缩变化都很小，两组间无差异(P＞0．05)；而含血清培养液(SCM)组的收缩变化非常大，与其它两组间具有极显著差异(P＜0．01)。结论：rHGF不能促进牙龈成纤维细胞重塑细胞外基质，为该细胞的HGF/SF无自分泌方式提供了证据。%AIM:To investigate the secretion mode of hepatocyte growth factor/scatter factor(HGF/SF) by gingivalfibroblasts in vitro. MEFHODS: Gingival fibroblasts were cultured, and the fibroblast populated collagen lattices (FPCLs) were farbricatad and divided into 3 groups. The diameter of the FPCL was measured from day 0 to day 7, and the degree of cell - mediated reorganization was estimated with three separate lattice diameter measurements. RESULTS: No differences inthe initial rate of lattice reorganizatin were obeerved between fibroblasts in serum - free median with and without 20 (or 40) ng/ml rHGF groups (P ＞ 0.05);culture in the presence of serum significantly stimulated lattice reorganization( P＜0.01 ).CONCLUSION: rHGF does not modulate fibroblast- mediated extracellular matrix reorganization. This study does not provide the evidence for the autocrine of HGF/SF by gingival fibroblasts.
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