目的：确认变形链球菌表面蛋白pac基因A区片段重组表达质粒载体pET-17b-A中的A区片段阅读框正确。方法：利用ABI310DNA自动测序仪进行DNA序列测定，并利用Internet进行联机检索，确定序列的构建正确与否。结果：测定了重组表达质粒载体pET-17b-A中的350个碱基序列，联机检索序列的构建正确。结论：重组表达质粒载体pET-17b-A中的A区片段阅读框正确。%AIM: To confirm and correct the open reading frame of pac gene'sA region in pET-17b-A. METHODS: pET-17b-A was sequenced by autosequence instrument ABI1310. The sequence was matched to the GenBank via Internet. RESULTS: The sequenced 350 bases matched the pac gene's A region after they were searched in GenBank. CONCLUSION: The open reading frame of pET-17b-A was correct.
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