慢性牙周炎牙龈组织细胞凋亡及凋亡相关蛋白Caspase-3的表达

摘要

目的:研究慢性牙周炎牙龈组织中细胞凋亡的发生情况和Caspase-3蛋白的表达,探讨其在慢性牙周炎病变发生中的意义.方法:应用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、免疫组织化学方法检测21例慢性牙周炎牙龈组织和21例健康牙龈组织中的细胞凋亡指数(apoptosis in-dex,AI)及Caspase-3蛋白的表达.结果:慢性牙周炎组牙龈组织中细胞凋亡指数明显高于正常对照组(P<0.05).与正常组相比,慢性牙周炎组牙龈组织中细胞caspase-3表达明显增强,两组间有显著性差异(P<0.05).结论:慢性牙周炎病人牙龈组织细胞发生凋亡,且通过激活细胞凋亡信号传导途径中的Caspase-3而导致慢性牙周炎发生.%AIM: To investigate whether apoptosis exist in gingival tissues of chronic periodontitis and toexamine the expression of Capase 3 in gingival tissues. METHODS: Gingival tissues from 21 periondontitis patients and 21 periodontally healthy individuals were collected, respectively. Apototic cells were detected by TUNEL staining and caspase-3 expression were determined by immunohistochemical staining SP method. RESULTS: The apoptosis index in gingival tissues of chronic periodontitis group was significantly higher than that in the normal group ( P < 0.05). Compared with the normal group, the expression of Caspase-3 was significantly stronger in chronic periodontitis group (P<0.05). CONCLUSIN: Cell apoptosis might play a role in the tissue destruction of chronic periodontitis.