bFGF对人根尖牙乳头干细胞成脂分化影响的研究

摘要

AIM: To investigate the effect of bFGF on the adipogenic differentiation of hunman stem cellsrnfrom the apical papilla (SCAP) in vitro. METHODS: SCAP was primarily cultured with enzyme-digest methods. Then SCAP was induced to differentiate to the adipogenic cells with or without bFGF (5ng/mL) in vitro. After 1 and 3 weeks, the induced cells were assessed with cell morphological observation, Oil Red-O staining and the expression of PPAR-72. RESULTS: The density of induced cell in experimental group was higher than that in control group after 3 weeks adipogenic induced. The induced cells were small and did not stretch to be longer in the experimental group. As compared to the control group, the expression level of PPAR-γ mRNA in the experimental group induced for 3 weeks was increased distinctly (P < 0. 05). The change of PPAR-γ mRNA expression was not observed after 1 week induced (P >0. 05). CONCLUTION: bFGF can increase the adipogenic differentiation potential of SCAP.%目的:研究碱性成纤维细胞生长因子(bFGF)在体外对人根尖牙乳头干细胞(stem cellsfrom apical papilla,SCAP)成脂分化的影响.方法:采用酶消化法获得原代SCAP,用含有5 ng/mL bFGF的成脂诱导液对其诱导培养不同时间,显微镜观察细胞形态变化、油红0染色观察SCAP成脂分化能力、RT-PCR检测PPAR-γ2 mRNA表达情况.结果:SCAP在含有5 ng/mL bFGF成脂诱导液中培养3周,细胞中脂滴形成数量多于对照组,而且细胞密度也相对较大,细胞体积小,未见拉长、变大等现象.RT-PCR检测显示,诱导培养1周时,实验组与对照组PPAR-γ2 mRNA表达无显著性差异(P＞0.05);培养3周时,实验组PPAR-γ2 mRNA表达量明显高于对照组(P＜0.05).结论:bFGF具有促进SCAP成脂分化的潜能.