五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响

摘要

AIM:To observe the effects of gallnut water extracts on collagen I synthesis of human dental pulp cells.METHODS:Cultured human dental pulp cells of passage 6 were treated with 5 μg/mL and 10 μg/mL of gallnut water extracts for 3 d respectively.Collagen I expression of the cells was detected by immunocytochemistry method.The quantitative data was obtained by HPIAS-1000 image analysis system.RESULTS:Collagen I protein was expressed in of both gallnut water extract treated dental pulp cells and control cells,and located mainly in cyto-plasm.Gallnut water extracts treatment dose-dependently increased collagen I protein expression (P<0.05).CON-CLUSION:Both 5 μg/mL and 10 μg/mL gallnut water extracts can enhance collagen I protein expression in human dental pulp cells,indicating that it is potential to promote dental pulp cells to differentiate into odontoblasts.%目的：观察五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响。方法：取第6代体外培养的人牙髓细胞分别与终末浓度为5、10μg/mL的五倍子水提取物共同培养3 d后，采用免疫组化染色法观察人牙髓细胞中Ⅰ型胶原的表达情况，并用HPIAS－1000图像分析系统进行定量分析。结果：对照组和不同浓度五倍子水提取物作用的实验组人牙髓细胞中均有Ⅰ型胶原的表达，其免疫反应物质多定位于胞浆。人牙髓细胞经5、10μg/mL浓度的五倍子水提取物作用后，其Ⅰ型胶原的表达水平均明显增高，其中以10μg/mL组的增高程度最大，各组间两两相比差异均有统计学意义（P＜0．05）。结论：5、10μg/mL五倍子水提取物均能明显促进人牙髓细胞中Ⅰ型胶原的表达；提示其具有促进牙髓细胞向成牙本质细胞分化的作用。