AIM:To compare the effects of dentin surfaces treated by different endodontic irrigation on the proliferation and differentiation of human dental pulp stem cell ( hDPSCs) . METHODS:The extracted healthy human teeth were cleaned and shaped using ProTaper and K-type file rotary instrumentation. The irrigation treatments investi-gated were 52. 5 g/L sodium hypochlorite (group A), 30 mL/L hydrogen peroxide and 52. 5 g/L sodium hypochlorite (group B), 170 g/L EDTA and 52. 5 g/L sodium hypochlorite (group C). hDPSCs were seeded on the treated dentin slices with the density of 2 × 105/mL. Cell adhesion and proliferation on the dentin slices were observed by SEM and MTT method. Cell differentiation was evaluated by ALP activity assay and Real-Time PCR. RESULTS:hDPSCs ad-hered well on all dentin slices. The cells in group C showed more proliferation higher ALP level and mRNA expression of OC, DSPP and DMP-1 than those in group A and B. CONCLUSION:Dentin slices treated by EDTA-root canal preparation can promote proliferation and odontogenic differentiation of DPSCs.%目的：：比较不同根管冲洗剂处理根管壁后对牙髓干细胞( hDPSCs)增殖及分化能力的影响。方法：用镍钛ProTaper和K锉预备新鲜健康离体牙,随机分为3组,A组：52.5 g/L次氯酸钠( NaClO)冲洗；B组：30 mL/L 过氧化氢液和52.5 g/L NaClO交替冲洗；C组：170 g/L EDTA和52.5 g/L NaClO交替冲洗。D组：为培养的hDPSCs,作为空白对照组。牙髓干细胞分别接种于各组牙本质中,扫描电镜( SEM)和MTT法分别观察细胞的粘附和增殖情况,并检测ALP的活性和矿化基因的表达。结果：A、B、C组hDPSCs均生长粘附良好。其中C组SEM下细胞数量最多,表现出最强的增殖能力；且该组细胞显示出较高的碱性磷酸酶活性和矿化基因表达水平。结论：经标准根管预备EDTA处理的根管壁可促进hDPSCs的增殖和向成牙本质细胞分化。
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