青春期牙龈炎牙龈卟啉单胞菌ra g位点基因分型与致病力分析

摘要

AIM:To investigate the distribution of Porphyromonas gingivalis(Pg)rag genotypes in adoles-cence gingivitis patients.METHODS:64 adolescent gingivitis patients (GI≥1,PD≥3 mm)and 56 gingivally healthy controls (GI=0,PD<3 mm)were included.Gingival crevicular fluid(GCF)was collected from all subjects. Pg and rag genotypes of Pg in GCF were detected by PCR method.RESULTS:The detection rate of Pg in GCF of the patients and healthy controls was 65.63%(42/64)and 33.93%(19/56)respectively(P<0.05).In Pg postive pa-tients(n=37 ),the rag genotypes in Pg positive samples was in the following order:rag -3 (16/37 ),rag -1 (9/37),rag-4 (7/37)and rrag-2 (5/37),in the controls(n=10)was rag-3(5/10),rag-2(2/10),rag-4 (2/10)and rag-1(1/10)(P<0.05).CONCLUSION:Various rag genotypes can be found in GCF of adolescent gingivitis patients.Rag-1 and rag-3 might have more close correlation with the development of adolescent gingivitis.%目的：分析不同rag基因型牙龈卟啉单胞菌在青春期牙龈炎中的分布状况。方法：分别选取青春期牙龈炎（GI≥1，PD≥3 mm）64例及牙周健康者（GI＝0，PD＜3 mm）56例作为研究对象，采用16S rDNA聚合酶链反应（PCR）法检测受试者龈沟液标本中牙龈卟啉单胞菌及致病岛rag 基因，分析其与临床指标之间的相互关系。结果：病例组病变位点和对照组牙龈卟啉单胞菌阳性检出率分别为65．63％（42／64）、33．93％（19／56）（P＜0．05）。牙龈卟啉单胞菌阳性患者（n＝37）的 rag 基因型检出率依次为 rag－3（16／37）、rag－1（9／37））、rag－4（7／37）和rag－2（5／37）。结论：青春期牙龈炎患者龈沟液中牙龈卟啉单胞菌存在rag基因多态性，其中rag－1和rag－3基因型牙龈卟啉单胞菌与青春期牙龈炎的发生发展关系密切。