siRNA干扰技术对沉默CKIP-1基因大鼠进展性牙周炎影响的研究

摘要

AIM:To study the silence of Casein kinase 2-interacting protein 1 (CKIP-1) gene by siRNA interference on the development of periodontitis in rats.METHODS:9 SD rats were randomly divided into 3 groups (n =3).The rats in group NC and group PC were treated by ligation and injection of LPS-PG at second maxillary molars for the establishement of periodontitis modelse.During the construction of the model,the rats in PC group were injected with CKIP-1 siRNA,and in NC group with saline as a control.The rats without treatment were used as the blank controls(BC goup).20 and 30 d after set-up of the experement Micro-CT and HE staining was performed to campare the damage of alveolar bone and the inflammatory response of the rats among the groups.RESULTS:The decrement of alveolar bone height in NC group was much more than that in PC group,alveolar bone absorption (mm):0.9606 ± 0.1112 vs 0.6759 ± 0.0514;alveolar bone volume percentage (％):0.5958 ± 0.014 vs 0.7509 ± 0.0355 (P ＜ 0.05).The alveolar bone absorption and the periodontal fiber sorting disorder was more obvious in the NC group than in the PC group.CONCLUSION:Silencing of CKIP-1 gene by siRNA interference can reduce the bone loss and inflammation in the rats with periodontitis.%目的:通过siRNA干扰技术沉默络蛋白激酶2-作用蛋白1(CKIP-1)基因,以观察其对大鼠牙周炎发生发展的影响.方法:取SPF级SD大鼠9只,并将其随机分为空白对照组(BC组)、阴性对照组(NC组)、阳性对照组(PC组)(n=3).其中NC、PC两组均采用双侧上颌第二磨牙细线结扎和注射LPS-PG的方法建立牙周炎模型,并在造模期间对PC组注射CKIP-1 siRNA干扰;NC组注射生理盐水作为对照.造模加干预4周后,分别对所有大鼠的双侧上颌骨进行Micro-CT扫描,分析其骨缺损高度和根分叉处牙槽骨体积的变化;然后再取其双侧上颌骨标本并制作近远中向牙周牙体组织联合切片,常规HE染色观察其牙槽骨破坏及牙周组织炎症反应情况.结果:Micro-CT扫描显示:NC组的骨缺损程度大于PC组,其牙槽骨吸收高度(mm)分别为0.9606±0.1112、0.6759±0.0514,根分叉处牙槽骨体积(％)分别为0.5958±0.014、0.7509±0.0355(P＜0.05).组织学观察显示:相比于PC组,NC组大鼠的牙槽骨吸收更明显,且牙周膜纤维排序紊乱.结论:通过siRNA干扰技术沉默CKIP-1基因后,可减少大鼠牙周炎模型的骨缺损破坏程度及炎症反应.