16S rRNA测序方法分析KSL抗菌肽口香糖对口腔细菌的抗菌效果

摘要

目的:应用16S rRNA测序方法研究KSL抗菌肽口香糖对口腔细菌的抗菌效果.方法:分别收集6名志愿者使用KSL抗菌肽口香糖前后的唾液,并提取总DNA进行16S rRNA测序分析,以比较使用KSL抗菌肽口香糖前后的口腔细菌的组成和丰度变化.结果:在门水平上,KSL抗菌肽口香糖对变异菌门、拟杆菌门和厚壁菌门的抑制作用比较明显(P<0.05);在属水平上,KSL抗菌肽口香糖对拟杆菌属、奈瑟氏菌属、嗜血杆菌、韦永氏球菌属、普氏菌属、卟啉单胞菌属、链球菌属的抑制作用比较明显(P<0.05).结论:KSL抗菌肽口香糖对口腔中的部分致病菌具有抑制作用.%AIM:To investigate the bactericidal effect of KSL antimicrobial peptides chewing gum on oral bacteria by 16S rRNA sequencing technique.METHODS:Saliva samples were collected before and after using KSL antimicrobial peptides chewing gum from 6 healthy subjects.Saliva samples′ DNA was extracted and the 16S ribosomal RNA gene clone library was constructed. The composition and abundance of bacteria before and after chewing the gum were analyzed.RESULTS:A total of 375 OTUs were obtained.At phylum level, KSL antimicrobial peptides chewing gum showed significant inhibiting effect on Proteobacteria, Bacteroidetes and Firmicutes(P<0.05).At genus level, the chewing gum showed significant inhibiting effect on Bacteroides, Neisseria, Haemophilus, Veillonella, Prevotella and Streptococcus(P<0.05).CONCLUSION:KSL antimicrobial peptides chewing gum has bactericidal effect on most pathogenic bacteria of dental caries and periodontal disease.