AIM:To study the level of IL-17 and IL-18 in gingival crevicular fluid (GCF) in patients with chronic periodontitis(CP).METHODS:20 patients with CP and 20 age-and gender-matched healthy subjects (aged 25-60 years) were included.GCF samples were collected from all subjects with Whatman 3M paper strip.Non-surgical periodontal therapy was performed for the patients.6 weeks after treatment,GCF samples were obtained again from the patients.Gingival index (GI),probing depth (PD),attachment loss (AL) and bleeding index (BI) were assessed.The levels of IL-17 and IL-18 in all the GCF samples was determined by ELISA.RESULTS:IL-17 and IL-18 levels were significantly higher in GCF of the patients than those in healthy subjects before and after treatment(P<0.05).In patients they were decreased significantly after periodontal therapy (P<0.05).All periodontal parameters were ameliorated after periodontal treatment(P<0.05).IL-17 and IL-18 levels were positively correlated with IL-17,IL-18 levels and periodontal destruction.CONCLUSION:IL-17 and IL-18 can be indexes for periodontal health.%目的:观察IL-17和IL-18在慢性牙周炎中的作用.方法:选择25~60岁慢性牙周炎患者和牙周健康者各20例,检查其牙龈指数(GI)、牙周探诊深度(PD)、附着丧失(AL)、出血指数(BI);用whatman3M滤纸条对患者进行龈沟液提取,牙周炎患者在基础治疗6~8周后再次提取龈沟液.酶联免疫吸附法(ELISA)检测龈沟液中IL-17和IL-18的水平,并进行统计学分析.结果:慢性牙周炎患者治疗前龈沟液中IL-17和IL-18的水平明显高于健康组(P<0.05);基础治疗后龈沟液中IL-17和IL-18的水平明显低于治疗前(P<0.05),但仍高于健康对照组(P<0.05).
展开▼
