Objective To screen high yield broad-spectrum antifungal antibiotic breeding CA-SD07 producing strain. Methods Two kinds of breeding methods ultraviolet radiation (UV) and high concentration of phosphate screening after ultraviolet radiation (UV+Pi) were conducted. Inhibition zone method, fermentation with flash shaking and with small fermentor were applied in screening high yield strains. Results 25 strains were isolated from UV group, 12 strains were isolated from UV+Pi group, during the first round flash shaking screening. The relative yield of antibiotic CA-SD07 of all these 37 strains increased by 50% or more than that of the original strain SD-07. 6 strains were screened during the second round flash shaking screening from the above 37 strains, their relative yield of antibiotic CA-SD07 increased by 100% or more than that of the original strain SD-07. Finally, 1 mutant strain was isolated by the fermentation characteristics: Its relative yield of antibiotic CA-SD07 stably increased by 100% or more than that of the original strain SD-07, and its fermentation period reduced more than 24 hours. Conclusion UV and UV+Pi got similar breeding results, high concentration phosphate screening did not significantly improve the antibiotic production of the isolated strains, but could improve the ratio of high antibiotic yield isolates to the whole ones. This suggested: In order to improve production of antibiotics, it needed improve the existing methods for high concentration phosphate screening.%目的 拟通过紫外线诱变结合耐高浓度磷酸盐筛选广谱抗真菌抗生素CA-SD07的高产菌株.方法 采用两种育种方式:紫外线诱变(UV),紫外线诱变后进行高浓度磷酸盐抗性筛选(UV+Pi).筛选方法采用抑菌圈法、摇瓶发酵和小型发酵罐发酵.结果 经过摇瓶初筛和复筛,从UV组和UV+Pi组共筛选到6株抗生素相对产量比原始菌株提高100％以上的高产菌.通过发酵罐发酵试验,选出1株抗生素相对产量稳定提高100％以上,且发酵周期缩短约24h的突变菌株.结论 UV+Pi与UV组育种效果接近,抗磷酸盐筛选并没有明显提高抗生素产量的作用,提示利用抗磷酸盐筛选高产菌株,需要在现行方法上进行改进才有可能实现.
展开▼
