核磁共振法测定硫酸依替米星含量

摘要

Qualitative and quantitative analysis of etimicin sulfate were reported by nuclear magnetic resonance(NMR).The 1H-NMR and 13C-NMR spectra of etimicin sulfate have been assigned by means of 1D and 2D spectroscopy including DEPT, COSY, HSQC and HMBC.On the basis of assignment, a novel approach was developed for the determination of purity of etimicin sulfate by proton-nuclear magnetic resonance (1H-NMR)using p-hydroquinone as internal standard.The result showed that the purity of etimicin was 59.19％, and its relative standard deviations(RSD) was 0.24％, this method was relative accuracy, precision, and ease of application.%通过核磁共振法对硫酸依替米星进行定性和定量分析.利用一维及二维核磁共振谱(DEPT、COSY、HSQC、HMBC),对H-NMR谱和C-NMR谱信号进行完整归属.在此基础上,采用氢核磁共振定量法,以对苯二酚为内标,测得硫酸依替米星中依替米星的含量为59.19%,RSD为0.24%,方法准确可靠,简便快速.