安莎类抗生素电喷雾多级质谱裂解规律的研究

摘要

Objective Electrospray ionization tandem mass spectrometric method was applied to the investigation of structural characterization of benzoquinoid ansamycins (geldanaymcin, herbimycin A) and naphthoquinoid ansamycins (rifamycin, rifapentin). Methods Based on electrospray ionization tandem mass spectrometric method under positive and negative mode. Results The fragmentation patterns and structural assignment of ansamycins in ESI-MSn were summarized. Conclusion These characteristic fragmentation patterns are useful in rapid structural identification, quantitative analysis and pharmacokinetic study of ansamycins compounds.%目的 研究苯安莎类抗生素(格尔德霉素、除莠霉素A)和萘安莎类抗生素(利福平、利福喷丁)的电喷雾多级质谱裂解规律.方法 采用电喷雾离子化源(Electron Spray Ionization Source,ESI源)多级质谱,极性检出模式为正/负离子模式(+/-MS).结果 初步归纳总结苯安莎类抗生素和萘安莎类抗生素电喷雾多级质谱裂解规律.结论 该质谱裂解规律可应用于安莎类抗生素的快速结构解析、定量分析和药代动力学研究.