苔藓内生菌Bacillus megaterium z12的分离、鉴定和抗菌活性筛选

摘要

Objective An endophytic bacteria zl2 was isolated from the leaf of Plagiochasma intermedium which has broad-spectrum antimicrobial activity. Methods According to the mophology, physiological and biochemical characteristics, combined with phylogenetic analysis, and the antimicrobial activity of zl2 was analyzed with 14 tested strains. Results The bacteria was identified as Bacillus megaterium. The results of inhibition experiments showed that zl2 strain had broad-spectrum antimicrobial activity to 7 target strains in different degrees, especially for Staphylococcus aureus and Candida albicans, Corticium sasakii were investigated by further tests with crude extracts of zl2 strain fermentation. Conclusion The strain is deserved to develop an agricultural antibiotics.%目的 从无纹紫背苔(Plagiochasma intermedium)叶片中分离筛选出一株具广谱抗菌活性的内生菌z12.方法 根据菌落和细胞形态、生理生化特性,以及16S rDNA序列对内生细菌z12进行了分析鉴定,并研究了它对14种供试菌株的抑制作用.结果 经分析鉴定该菌属于巨大芽孢杆菌(Bacillus megaterium).抑菌实验表明,内生细菌z12对7种供试菌都有不同程度的抑制作用,对金黄色葡萄球菌(Staphylococcus aureus)和白念珠菌(Candida albicans)的抑制作用较强.研究了z12菌株粗提物对水稻纹枯病原菌(Corticium sasakii)的抑制作用.结论 该菌株具有开发成农用抗生素的价值.