细胞外调节蛋白激酶信号通路在体外诱导神经干细胞向施万细胞分化中的作用

摘要

Objective: To study the effect of ERK pathway on the differentiation of rat neural stem cells into Schwann cells.Methods: Neural stem cells (NSCs) were cultured in vitro and were induced to differentiate into Schwann cells by adding a mixture of reagents into the medium. Cells were divided into control group cultured in the induction media and experimental group cultured in the mixture of NSCs media and inhibitor of ERK pathway. S100- and P75-positive ceils were labeled by double-labelling immunofluorescence. Results: After the administration of induction reagents, the level of phosphorylatedERK1/2 was elevated; Three weeks later, S100- and P75-positive cells were observed. The level of phosphorylated-ERK1/2 in the experimental group was decreased with administration of U0126. Schwann cells in the ERK group were significantly less than the control group. Conclusion: ERK signal pathway plays an important role in the induction of NSCs into Schwann cells.%目的:探讨细胞外调节蛋白激酶(ERK)信号转导通路在体外诱导大鼠海马神经干细胞向施万细胞分化中的作用.方法:体外培养大鼠海马神经干细胞,向培养液中添加混和诱导剂诱导神经干细胞向施万细胞分化.将培养细胞分为2组,对照组采用含诱导剂的培养液进行培养,免疫印迹法检测ERK磷酸化水平,实验组中在此基础上添加ERK通路抑制剂U0126,免疫荧光双标染色计数胶质细胞特异性标记物S100及P75阳性细胞比例.结果:加入诱导剂后,磷酸化ERK1/2水平上升;3周后,可见P75、S100阳性的施万细胞.加入通路抑制剂后,实验组的ERK磷酸化水平逐渐降低.与对照组相比,实验组的施万细胞所占百分比明显减少.结论:ERK通路在神经干细胞向施万细胞的诱导分化中起重要作用.