miR-155在子宫内膜癌组织中的表达及其临床意义

摘要

背景与目的:MicroRNAs是一类19～25 bp内源非编码的小分子RNA,能通过靶向抑制基因的转录和翻译水平.本研究旨在探讨hsa-miR-155(Homo sapiens miR-155,miR-155)在子宫内膜癌患者组织中的表达及其临床意义.方法:收集2007年9月-2009年12月子宫内膜癌患者手术标本66例,癌旁组织标本作为正常对照,并取其表达水平为1,应用原位杂交(in situ hybridization,ISH)及实时荧光定量PCR (real time quantity PCR,qRT-PCR)检测子宫内膜癌患者组织及癌旁组织中miR-155的表达水平,miR-155表达与子宫内膜癌临床病理参数及临床分期之间的关系,并分析miR-155表达与患者预后关系.结果:ISH结果显示,在66例子宫内膜癌组织中miR-155阳性表达率为84.85％,与癌旁对照组18.18％相比,差异有统计学意义(x2=58.72,P＜0.01)；qRT-PCR结果显示,临床Ⅰ/Ⅱ、Ⅲ/Ⅳ子宫内膜癌患者组织miR-155表达水平为(4.54±0.55)、(7.93±0.88)倍,差异有统计学意义(t=13.51,P＜0.01)；有、无盆腔淋巴结转移子宫内膜癌患者组织miR-155的表达水平分别为(9.56±0.86)、(5.49±0.47)倍,差异有统计学意义(t=15.12,P＜0.01)；高、中、低分化子宫内膜癌组织中miR-155表达水平分别为(6.42±0.63)、(6.97±0.59)、(6.27±0.46)倍,差异均无统计学意义(t=0.412,P＞0.05)； Kaplan-Meier生存分析显示,miR-155表达越高患者生存率越低(P=0.001).结论:miR-155与子宫内膜癌的发生、进展及淋巴结转移等密切相关,对子宫内膜癌潜在的治疗靶点及预后判断具有重大意义.%Background and purpose: MicroRNAs are 19- to 25-nucleotide noncoding RNA molecules that regulate gene expression at the level of transcription and translation. This study investigated the expression of the miR-1SS in tissue of endometrial cancer and its clinical significance. Methods: We collected the 44 cases tissue specimens and paracancerous tissues from September 2007 to December 2009, ISH and qRT-PCR was used to detect the miR-155 expression in those specimens. Results: ISH was used to detect the positive expression rate of the miR-155 was 18.18% in control and 84.85% in endometrial cancer. The real time quantity PCR detect the the expressions of the miR-155 were (4.54±0.55), (7.93±0.88) times in stage I - II and ID-1V endometrial cancer, respectively, the difference was statistically significant (f<0.01), the expressions of miR-155 were (9.56±0.86), (5.49±0.47) times in endometrial cancer of lymph node metastasis and without lymph node metastasis. The difference was statistically significant (P<0.01). The expression of miR-155 were not associated with differentiation degree, the difference was not statistically significant(P>0.05). The higher mortality with the higher expression of miR-155 from Kaplan-Meier survival curves. Conclusion: miR-155 may play an important role in the carcinogenesis, and metastasis of endometrial cancer, and it could be an indicator in the diagnosis and prognosis of endometrial cancer. miR-155 can be used as a potential target of therapy and a predictive biomarker.