背景与目的:鼻咽癌放射治疗后的局部复发和远处转移是患者死亡的主要原因之一.姜黄素(curcumin)是从姜科姜黄属植物姜黄(Curcuma longa)根茎中提取的一种酚性色素,具有抗菌、抗肿瘤及抗氧化作用.本研究旨在探讨姜黄素对人鼻咽癌CNE-2Z细胞侵袭和转移的影响,并探讨其可能的机制.方法:以10、20、40和80 μ mol/L姜黄素分别处理CNE-2Z细胞24、48 h后,MTT法检测其对细胞的生长抑制作用.应用Transwell小室进行人工重组基底膜(matrigel)侵袭和运动实验,观察姜黄素对CNE-2Z细胞侵袭和转移的影响.RT-PCR和Western blot分别检测不同浓度姜黄素作用后细胞中表皮生长因子受体(epidermal growth factor receptor,EGFR)表达水平的变化.结果:应用姜黄素处理后,人鼻咽癌CNE-2Z细胞生长受到抑制,且作用呈时效-量效依赖关系；同时细胞侵袭和迁移能力明显降低；EGFR基因和蛋白表达亦明显减弱.结论:姜黄素能够减弱人鼻咽癌CNE-2Z细胞的体外侵袭和转移能力,其机制可能与降低EGFR的表达有关.%Background and purpose: The main causes of nasopharyngeal carcinoma patient's death are loco-regional recurrence and distant metastasis after radiation therapy. Curcumin, a natural compound extracted from rhizomes of curcuma species, has been shown to possess potent anti-inflammatory, anti-tumor and anti-oxidative properties. This study aimed to explore the effects of curcumin on invasion and metastasis of human nasopharyngeal carcinoma CNE-2Z cells and to investigate the possible mechanism. Methods: CNE-2Z cells were treated with 10,20, 40 and 80 umol/L curcumin for 24 and 48 h, respectively. Proliferation of cells was measured by MTT assay. Invasion and metastasis of cells were evaluated with Transwell chamber. The expression of epidermal growth factor receptor (EGFR) was detected by RT-PCR and Western blot. Results: After being treated with curcumin, the proliferation of CNE-2Z cells was inhibited in a time and dose-depending manner, the invasion and metastasis of cells were also inhibited, and the expression level of EGFR was decreased. Conclusion: Curcumin could decrease the invasion and metastasis of human nasopharyngeal carcinoma CNE-2Z cells possibly by inhibiting the expression of EGFR.
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