成纤维细胞激活蛋白对卵巢癌细胞增殖、迁徙和侵袭的影响

摘要

Background and purpose: Activated fibroblasts of tumor stroma can express fibroblast activation protein (FAP). The purpose of this research was to study the effect of FAP in the proliferation, migration and invasion of ovarian cancer cells in vitro. Methods: Human ovarian cancer cell line HO-8910PM was cultured in vitro and was treated with different concentrations of FAP(30, 100 and 300 pmol/L), then examined the function of FAP in the proliferation, migration and invasion of HO-8910PM by the colorimetric MTT Assay, Wound-Healing Assay and Transwell Assay. Results: Colorimetric MTT Assay and the curve of reproduction of cells showed that FAPa could increase HO-8910PM cell proliferation in a duration- and dose-dependent manner; Wound-Healing Assay confirmed that FAPa could promote the migration of the ovarian cancer cells, especially the function of 100 pmol/L group (P<0.01); Transwell Assay certified that FAPa could promote the invasion of the ovarian cancer cells, especially the function of 100 pmol/L group (P<0.01). Conclusion: FAP may induce the ovarian cancer cells proliferation, invasion and migration.%背景与目的:肿瘤间质活化的成纤维细胞能表达成纤维细胞激活蛋白(fibroblast activation protein,FAP),本研究通过体外实验研究FAP在卵巢癌细胞增殖、侵袭和迁徙过程中的作用.方法:人类卵巢癌细胞系H0-8910PM体外培养,加入不同浓度的FAP(30、100和300pmol/L)处理,用MTT法检测FAP对H0-8910PM增殖的影响；细胞划痕实验检测FAP对HO-8910PM迁徙能力的影响；Transwe11侵袭实验来研究FAP对H0-8910PM侵袭能力的影响.结果:MTT法及细胞生长曲线显示FAP对卵巢癌细胞有促增殖作用,并呈时间、剂量依赖性；细胞划痕试验结果显示,不同浓度的FAP对卵巢癌细胞系的迁徙均有促进作用,以100pmol/L组最为明显(P＜0.01)；Transwe11侵袭实验显示FAP对卵巢癌细胞有促侵袭作用,以100pmol/L组最为明显(P＜0.01).结论:FAP具有促进卵巢癌细胞的增殖、迁徙和侵袭的作用.