Background and purpose: It has been an effective way by FISH to examine the amplification of the human telomerase RNA component (hTERC) gene in screening of early cervical cancer, however, the detection accuracy is yet to be evaluated. The aim of this study was to analyze the relationship of telomerase gene amplification and expression and HPV infection between minority and Han women from two regions in Guizhou Province, China. Methods: By investigating the amplification of hTERC, the expression of human telomerase reverse transcriptase (hTERT), HPV 16/18 E6, and HPV 16 E7 with FISH or immunohistochemistry, the differences were analyzed and compared in cevical cancer tissues between minority women (including Miao, Dong, and Tujia) and Han women from Tongren and Southeast Qian regions in Guizhou Province, China. Results: The results showed that no statistical significances were reached between minority and Han women in terms of the amplification of hTERC and the expression of hTERT, E6 or E7 (P>0.05). In both minority and Han women, there was no significant difference betweenthe amplification of hTERC and the expression of hTERT (.P>0.05), but a high statistical significance was reached between the expression of HPV16/18 E6 and the amplification of hTERC or the expression of hTERT (P<0.01). These data demonstrated that the infection of HPV 16/18 may be one of the major reasons that induce the amplification of hTERC and the expression of hTERT in cevical tissues. Conclusion: The detection of HPV 16/18 E6 and hTERT combined with the copy number examination of hTERC in cervical tissue may increase the accurate screening rate of cervical cancers in both minority and Han women in Guizhou Province, China.%背景与目的:用FISH检测人端粒酶RNA组分(human telomerase RNA component,hTERC)扩增是筛查宫颈组织早期癌变的有效方法,但是其检测结果的准确率尚不清楚.本研究旨在分析贵州省部分地区少数民族与当地汉族妇女宫颈癌中端粒酶基因扩增和表达与HPV感染的相关性.方法:用FISH检测hTERC基因的扩增,结合免疫组化检测端粒酶反转录酶(human telomerase reverse transcriptase,hTERT)、HPV16/18 E6抗原和HPV16 E7抗原的表达,分析和比较了贵州省部分地区少数民族(苗族、侗族和土家族)与汉族宫颈癌患者之间的扩增或表达的差异.结果:少数民族与汉族之间在hTERC扩增,hTERT、E6和E7表达上差异无统计学意义(P＞0.05):在少数民族和汉族中,hTERC基因的扩增与hTERT表达差异无统计学意义(P＞0.05),但HPV16/18感染与hTERC扩增或与hTERT表达之间具有显著相关性(P＜0.01).说明宫颈癌中,HPV感染可能是导致hTERC扩增与hTERT高表达的主要原因之一.结论:检测HPV16/18 E6和hTERT表达,并结合测定hTERC扩增可提高贵州省部分地区少数民族以及汉族妇女宫颈癌筛查的准确率.
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