背景与目的：长期以来，雌激素受体(estrogen receptor，ER)阳性乳腺癌患者对内分泌治疗耐药是临床上棘手的难题。目前研究表明，雌激素效应环指蛋白(estrogen-responsive finger protein，Efp)和polo样激酶3(polo-like kinase 3，Plk3)的表达与乳腺癌发展具有密切关系。该研究旨在探讨ER阳性乳腺癌中Efp和Plk3蛋白表达相关性，了解Efp和Plk3表达变化在耐药中的作用。方法：应用免疫组织化学SP法检测74例ER阳性乳腺癌组织中Efp和Plk3蛋白表达情况，结合临床资料进行分析。并采用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)及蛋白[质]印迹法(Western blot)检测ER阳性乳腺癌MCF-7细胞中Efp和Plk3的基因和蛋白表达变化。结果：74例ER阳性乳腺癌组织中Efp和Plk3蛋白表达与患者的临床病理特征未见任何关系(P>0.05)，其中有51例(68.9%)患者Efp表达阳性和23例(31.1%)患者Plk3表达阳性，χ2检验分析结果显示，ER阳性乳腺癌组织中Efp和Plk3具有显著的负相关关系(χ2=8.837，P<0.05)。RTFQ-PCR检测结果显示，MCF-7细胞经雌激素刺激后Efp mRNA的表达显著增加，而Plk3 mRNA的表达无明显变化。Western blot检测结果显示，MCF-7细胞经雌激素和MG132刺激后，Efp的蛋白表达较MG132组显著增加，而Plk3蛋白表达明显下降。结论：在ER阳性乳腺癌患者中Efp和Plk3的蛋白表达呈负相关，Efp高表达可促进Plk3的蛋白降解，对内分泌耐药过程的形成可能产生一定影响。%Background and purpose:Estrogen receptor (ER)-positive breast cancer always presents a dilemma for resistance to endocrine therapy in a long time. The recent studies showed that the expression of estrogen-responsive ifnger protein (Efp) and polo-like kinase 3 (Plk3) had a close relationship with breast cancer development. This study was to explore the expression correlation between Efp and Plk3 in ER-positive breast cancer in order to understand the inlfuence of Efp and Plk3 on the drug resistance.Methods:The expression of Efp and Plk3 in 74 cases of ER-positive breast cancer was detected by SP immunohistochemistry. The clinical signiifcance was then analyzed. Real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were used to detect the expression of Efp and Plk3 in ER-positive MCF-7 cells.Results:No signiifcant relationship was found between Efp and Plk3 expression and the clinicopathological features of 74 cases of ER-positive breast cancer (P>0.05). The number of cases whose Efp showed positive expression was 51 (68.9%), while the number of cases whose Plk3 showed positive expression was 23 (31.1%). Chi-square test analysis showed the expression of Efp and Plk3 was negatively correlated in 74 cases of ER-positive breast cancer (χ2=8.837,P<0.05). The result of RTFQ-PCR showed that the expression of Efp mRNA in MCF-7 cells was up-regulated by estrogen stimulation, whereas Plk3 mRNA was not changed. The result of Western blot showed that the expression of Efp protein in MCF-7 cells was increased by estrogen and MG132 stimulation, whereas Plk3 protein was decreased.Conclusion:The expression of the Efp protein is negatively correlated with Plk3 protein in ER-positive breast cancer. High expression of Efp may be involved in the resistance to endocrine therapy.
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