前列腺等离子电切组织α1-肾上腺素受体的分布情况研究

摘要

目的 了解前列腺无炎症增生组织和合并炎症增生组织α1肾上腺素受体(α1-AR)的分布情况.方法 选取30例良性前列腺增生(BPH)患者,收集经尿道前列腺等离子电切术后前列腺增生组织,采用双免疫组织化学法,观察前列腺增生组织中α1-AR 3种亚型的染色光密度(OD)和切片组织炎症细胞的浸润情况.结果 12例前列腺标本切片未发现炎症细胞浸润,18例前列腺标本切片有炎症细胞浸润.前列腺无炎症增生组织α受体亚型α1A-AR、α1B-AR、α1D-AR的OD值与合并炎症增生组织比较,差异无统计学意义(P＞0.05).结论 前列腺无炎症增生组织与合并炎症增生组织的α受体亚型α1A-AR、α1B-AR、α1D-AR含量无差异.%Objective To investigate the distribution of alpha 1 adrenergic receptor (α1-AR) in benign prostatic hyperplasia tissues with or without inflammation after transurethral plasmakinetic resection of prostate (PKRP).Methods The prostatic tissues of benign prostatic hyperplasia were collected from 30 patients after PKRP.The distribution of α1-AR was measured by immunohistochemistry using specific monoclonal antibodies of α1-AR subtypes.The inflammatory cell infiltration of prostatic tissues was observed at the same time.Results There were no inflammatory cells in 12 prostatic tissues.There were inflammatory cells in 18 prostatic tissues.The mean optical density (OD) of α1A-AR,α1B-AR and α1D-AR in the benign prostatic hyperplasia tissues was not statistically different between the non-inflammation group and the inflammation group (P ＞ 0.05).Conclusions The content of α1A-AR,α1B-AR and α1D-AR in the benign prostatic hyperplasia tissues with inflammation is not significantly different from that in the benign prostatic hyperplasia tissues without inflammation.