糠秕马拉色菌皮肤感染模型的构建

摘要

Objective:To construct an in vitro model of tissue-engineered skin for Malassezia furfur infec-tion. Methods:The tissue engineered dermis was used to construct with primary cultured fibroblasts and bo-vine collagen mixed solution. And then, keratinocytes and melanocytes were seeded on the surface of the engi-neered dermis with the method of air-liquid interface culture. The suspension of Malassezia furfur was inocula-ted with the pigmented tissue-engineered skin. At different time points, HE staining and PAS staining were performed. Results:The structure of the tissue engineered skin was intact and compact, with 8-10 cell layers. Blastospores of Malassezia furfur were only seen in the upper of epidermis over time through the hematoxylin-eosin staining and periodic acid-schiff staining. Conclusion:Malassezia furfur infected tissue-engineered skin was successfully established, which can be used for a study models of Malassezia furfur infection in vitro.%目的：：构建糠秕马拉色菌的体外组织工程皮肤感染模型。方法：利用成纤维细胞及牛胶原混合液构建组织工程真皮，采用气液界面培养方法在组织工程真皮表面培养角质形成细胞和黑素细胞；糠秕马拉色菌悬液感染组织工程皮肤，在不同时间点，利用HE染色及PAS染色观察组织工程结构的改变及糠秕马拉色菌侵入深度的变化。结果：构建的组织工程皮肤结构紧密，分化层数达8~10层。随着糠秕马拉色菌感染时间的延长，组织工程皮肤的基本结构不被该菌破坏，芽生孢子密度随着时间延长而增加，对于组织工程皮肤的侵袭仅限于表皮浅层。结论：糠秕马拉色菌的组织工程皮肤感染模型可较好的代表糠秕马拉色菌在体感染情况，是马拉色菌相关疾病较好的体外研究工具。