屋尘螨变应原 Derp1对人肥大细胞HMC－1释放类胰蛋白酶的影响

摘要

Objective: To determine the effect of house dust mite Derp1 on the secretion of typtase from human mast cell line HMC-1. Methods:After cultured HMC-1 cells were incubated respectively with Derp1 5 μg/mL and Derp1 5 μg/mL+PAR2 antagonist ( FSLLY) 100 μmol/L. The level of typtase from HMC-1 cells was detected by ELISA. The degranulation of HMC-1 cells was observed under microscope. Results:The levels of the typtase from HMC-1 cells in culture medium group, Derp1 group and Derp1 combined FSL-LY group were 31.12±3.60 ng/mL, 70.40±1.55 ng/mL and 56.72±2.66 ng/mL, respectively. The degranula-tion rates in Derp1 group and Derp1 combined FSLLY group were 66. 67% and 16. 67%. Conclusion: The PAR2 receptor can be activated by house dust mite Derp1, which results in typtase secretion of the human mast cell line HMC-1.%目的：：明确屋尘螨变应原Derp1对人肥大细胞系HMC－1释放类胰蛋白酶的影响。方法：培养后的HMC－1细胞分为单纯培养液组、5μg/mL Derp1组和5μg/mL Derp1＋100μmol/L PAR2拮抗剂( FSLLY)组，用ELISA方法检测各组类胰蛋白酶水平，显微镜观察HMC－1细胞脱颗粒率。结果：培养液组，Derp1组和Derp1＋FSLLRY组中类胰蛋白酶水平分别为31.12±3.60 ng/mL、70.40±1.55 ng/mL和56.72±2.66 ng/mL。 Derp1组和Derp1＋FSLLRY组肥大细胞脱颗粒率分别为66.67％和16.67％。结论：屋尘螨变应原Derp1可以通过激活人肥大细胞HMC－1表面PAR2受体引起肥大细胞脱颗粒释放类胰蛋白酶。