辛伐他汀对白癜风氧化应激模型中角质形成细胞趋化因子分泌的影响

摘要

Objective:To determine the effect of simvastatin on the expression of CXCL9, CXCL10, CX-CL11 and CCL22 in H2O2-treated primary human keratinocytes (KC). Methods: KC in the experimental group was pre-treated with different concentration of simvastatin ( 0. 1 μmol/L, 0. 5 μmol/L and 1. 0 μmol/L) , and then, exposed to 1.0 mM H2 O2 for 24 h. KC in the H2 O2 group was treated with 1.0 mM H2 O2 only. The expression levels of mRNA and protein of CXCL9, CXCL10, CXCL11 and CCL22 were detected by Real-time PCR, Western blot and ELISA. Results:The expression levels of mRNA and protein of CXCL9, CX-CL10 and CXCL11 in the experimental group were higher than those in H2 O2 group, and the level of CCL22 was lower than that in H2 O2 group. Conclusion: Simvastatin can influence the levels of CXCL9, CXCL10, CXCL11 and CCL22 through regulating oxidatively stressed KC.%目的:明确辛伐他汀对氧化应激下人原代角质形成细胞(KC)分泌趋化因子CXCL9、CX-CL10、CXCL11和CCL22的影响.方法:常规培养KC,H2 O2组给予1 mM H2 O2模拟白癜风KC氧化应激模型,实验组予不同浓度辛伐他汀(0.1μmol/L、0.5μmol/L、1.0μmol/L)预处理后加入H2O2;采用Real-time PCR、ELISA及Western blot检测CXCL9、CXCL10、CXCL11和CCL22的mRNA表达及蛋白分泌.结果:辛伐他汀组CXCL9、CXCL10、CXCL11水平低于H2O2组,CCL22水平高于且呈H2O2组,呈剂量依赖方式.结论:辛伐他汀能通过应激的角质形成细胞调控分泌Th1型趋化因子CXCL9、CXCL10、CXCL11及CCL22的水平.