High-throughput sequencing was used to parse the microbial community of activated sludge in an in-situ sludge reduction process. Microbial community structure shifted dramatically as the result of bioaugmentation. The abundance of Lactobacillus and Acetobacterium increased significantly in bioaugmentation group. Community diversity indices including Simpson index, Shannon index and Pielou index rised at different degrees in anaerobic zone of bioaugmentation group. Cluster analysis indicated that microbial communities were obviously grouped into three clusters in chronological order. In anaerobic zone of bioaugmentation group, the community structure shifted more obviously over time. Through principal coordinate analysis, communities of bioaugmentation group and control group were grouped into two distinct areas. Synergistic efforts of bioaugmentation and DO reduction were the driving forces of community shift in bioaugmentation group, while DO reduction was the driving force in control group. Canonical correspondence analysis showed that pH, water temperature and DO were the dominant environmental factors, which affected the microbial community structure significantly.%利用高通量测序技术对微生物强化原位污泥减量工艺中微生物群落进行解析.结果表明,外源菌剂的投加改变了活性污泥中微生物群落结构,菌剂的有效成分乳杆菌属和醋酸杆属在强化组中含量显著增加.科水平上的群落多样性分析显示：强化组厌氧区 Siompson 指数、Shannon 指数和 Pielou 指数均不同程度升高,群落多样性增加.聚类分析显示,微生物群落按时间顺序明显聚为3簇,强化组厌氧区微生物群落随时间发生较大演变.主坐标分析显示,强化组和对照组微生物群落明显聚为2类,其厌氧区群落分别按照不同的方向演变,微生物强化和DO 降低的协同作用是强化组群落演变驱动力,DO 降低是对照组群落演变驱动力.典范对应分析显示显著影响微生物群落结构的环境因子依次为 pH 值、水温和 DO.
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