With increasing depletion of conventional fossil energy,more and more attention has been paid to biodiesel as a renewable alternative energy source. Microalgae oil extraction is a key part in promotion and the application of biodiesel. In this study,microalgae oil was extracted with organic solvents,and oil extraction yield of microalgae was investigated under different conditions. Microalgae oil extraction yield was especially studied by successive use of two organic solvents,methanol and petroleum ether. Temperature,liquid-material ratio and extraction time affected extraction rate,and the use of methanol and petroleum ether increased algae oil extraction yield significantlyvia two steps. Extraction yield was 58.71% when organic solvent was petroleum ether,liquid-material ratio was 15mL/g,temperature was 45℃and extraction time was 5h. Moreover,extraction yield could be increased to 87.90% when methanol and petroleum ether were used successively in two steps,while temperature could be lowered to 35℃ under the same conditions of liquid-material ratio and extraction time.%在传统化石能源日益枯竭的趋势下，微藻生物柴油作为第三代绿色可再生的替代型能源越来越受到人们的重视。在微藻生物柴油的产业链上，油脂的提取是影响其推广应用的一个关键环节。本文实验利用有机溶剂提取微藻油脂，探究在不同的条件下微藻油脂的提取效果，并特别研究了先后使用甲醇和石油醚两种有机溶剂对微藻油脂提取率的影响。研究结果表明：温度、液料比、浸提时间对提取效率都有一定的影响，并且使用甲醇和石油醚两种溶剂分步提取时会使微藻油脂提取率明显提高；在液料比为15mL/g、提取温度为45℃、提取时间为5h时，使用石油醚作为提取剂的提取率为58.71%；使用甲醇溶剂提取后再使用石油醚提取时，在液料比和提取时间相同的条件下，温度为35℃时提取率即可达87.90%
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