炼油废水中好氧反硝化菌的筛选及降解特性

摘要

In order to improve the denitrification efficiency of the bioreactor of refinery wastewater treatment plant,an aerobic denitrifier AD10 was isolated from the activated sludge of a petrochemical wastewater treatment plant in wuhan city. It was identified as a member of the genusPseudomonas sp. according to its morphyological and physiological properties and 16S rDNA gene sequence analysis. Through the single factor experiment investigations,the optimum denitrification conditions were: sodium succinate as carbon source,C/N 14,temperature 30℃,initial pH 6.0,rotation speed 200 r/min. Under these conditions,the nitrate remove efficiency of AD10 was 97.2% when it was cultivated in 556.81 mg/L nitrate for 72h,and it could tolerate at least 654 mg/L of nitrate nitrogen. Culturing after 11.5h,AD10 show the NO3–-N and TN removal rate of 95.8% and 93.9%,when the concentration of NO3--N and TN were 140.31mg/L and 141.62mg/L,respectively. AD10’s denitrification rate is higher than most of the aerobic denitrifying bacteria have been found. During this process,only a very small amount of NO2–-N (≤2.30mg/L) was detected,indicating a complete denitrification.%为了提高炼油废水生物处理装置的反硝化性能，从武汉石化污水处理厂的活性污泥中筛选出一株好氧反硝化菌AD10，经生理生化及16S rDNA序列分析，将其鉴定为假单胞菌属。通过单因素实验研究了该菌的最适培养条件：以丁二酸钠为碳源，C/N=14，温度30℃，初始pH=6.0，摇床转速200r/min。在此条件下，ρ(NO3−-N)=556.81mg/L时72h的去除率为97.2%，且耐受的硝酸盐氮质量浓度达到654.00mg/L。菌株AD10培养11.5 h，对ρ(NO3−-N)=140.31mg/L和ρ(TN)=141.62mg/L的去除率分别为95.8%和93.9%，其反硝化速率高于大部分已发现的好氧反硝化菌，检测到的ρ(NO2–-N)≤2.30mg/L，未出现明显的积累，菌株AD10能够进行完全反硝化。